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. Block diagram of an HPLC–MS. A 3 component combination enters the HPLC. When component A elutes from your column, it enters the MS ion source and ionizes to variety the guardian ion and a number of other fragment ions.The present flowing involving the working electrode as well as the auxiliary electrode serves given that the analytical signal.
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Incorporate a known degree of the antidepressant protriptyline, which serves as an internal regular, to each serum sample and to each external common. To remove matrix interferents, go a 0.5-mL aliquot of every serum sample or typical through a C18 stable-section extraction cartridge. After washing the cartridge to eliminate the interferents, elute
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